PREPARATIVE SEPARATION AND PURIFICATION OF BIONDNOID I AND MAGNOLIN FROM THE CHINESE MEDICINAL PLANT FLOS MAGNOLIAE BY HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY

Abstract

A preparative high-speed counter-current chromatography (HSCCC) method for separation and purification of biondnoid I and magnolin from the Chinese medicinal plant Flos Magnoliae was successfully established by using light petroleum-ethyl acetate-methanol-water (3:7:4:6, v/v) as the two-phase solvent system. From 500 mg of crude extracts of Flos Magnoliae 48 mg of biondnoid I with a purity of 99.3% and 135 mg of magnolin with a purity of 98.7% were separated in a one-step separation. The structures of biondnoid I and magnolin were identified by ¹H-NMR.

Keywords:

• biondnoid I
• Flos Magnoliae
• high-speed counter-current chromatography
• magnolin
• purification
• separation

ACKNOWLEDGMENT

This work was supported by Natural Science Foundation of Shandong Province (No 2009ZRB01753), Key National Science & Technology Specific Projects of China (2010ZX09401-302-5-12), and The Project of Taishan Scholarship of Shandong Province.