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Abstract
A rapid, sensitive, and simple high performance liquid chromatography photo diode array mass spectrometry (HPLC-PDA-MS-MS) method was developed for the simultaneous determination of nine compounds, namely, psoralen (furocoumarin), daidzein, genistein (isoflavonoids), daidzin and genestin (isoflavone glycosides), biochanin A (methoxylated isoflavone), and quercetin, kaempferol, and rutin (flavonols). Chromatographic separation of these nine molecules was performed on RP C18 column within 20 min. Elution was accomplished by the application of trifluoroacetic acid (0.05%) in water and methanol in gradient system with flow rate of 1.0 mL/min. PDA and mass spectrometry was employed for qualitative and quantitative analysis. The method is economical in terms of the time expended and the amount of solvent used for each analysis. The calibration curves were linear and ranged between 0.994 to 0.999; limit of detection (LOD) and quantification (LOQ) ranged between 1.02 to 1.18 and 2.23 to 3.34 and recovery ranged between 91.76% to 97.41%. The validated method was successively used to separate and quantitative measurement of these compounds in plant parts of Psoralea corylifolia. The developed method may be used in the quality control and standardization of plant extracts as well as herbal drugs and formulations. Supplemental materials are available for this article. Go to the publisher's online edition of Journal of Liquid Chromatography & Related Technologies to view the free supplemental file.
Notes
a The retention times (RT) in minutes are the mean values of 10 replicates.
b correlation coefficient.
YR ˭ Young Root; MR ˭ Mature Root; MF ˭ Mature Fruit; ML ˭ Mature Leaves; YL ˭ Mature Leaves; Twg = Twig.
A ˭ Acid Hydrolysis; B ˭ Without Hydrolysis; ND ˭ Not Detected.