QUALITATIVE AND QUANTITATIVE ANALYSIS OF CHLORANTRANILIPROLE AND FLUBENDIAMIDE IN SOLUBLE CONCENTRATE FORMULATIONS BY HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY

Abstract

A method was developed for the qualitative analysis of active ingredients in soluble concentrate (SC) formulations of chlorantraniliprole (Coragen 18.5 SC) and flubendiamide (Fame 480 SC). Known concentrations of the reference grade standards and the soluble concentrate of chlorantraniliprole and flubendiamide dissolved in acetonitrile were applied on silica gel 60 F₂₅₄ HPTLC plates using a Camag-Hamilton Linomat Syringe (100 μL). These plates were developed in horizontal chambers with ethyl acetate and methanol as mobile phase for chlorantraniliprole and flubendiamide, respectively. The amount of active ingredients present in formulations of chlorantraniliprole and flubendiamide were estimated by densitometry in a single beam, single wavelength reflectance mode at 270 and 252 nm, respectively. Calibration curves of these insecticides were linear in the range of 50–2000 ng and the correlation coefficients for the calibration equation were 0.998 and 0.996 for chlorantraniliprole and flubendiamide, respectively. The method has been validated by further analysis using high performance liquid chromatography (HPLC) with photo diode array (PDA) detector, and results were comparable. The method was found to be reproducible and convenient for quantitative analysis of these compounds. The proposed HPTLC method has its application for quality control and determination of the shelf life of commercial formulations of pesticides.

Keywords:

• active ingredient
• chlorantraniliprole
• flubendiamide
• high performance liquid chromatography
• high performance thin layer chromatography
• soluble concentrate

Notes

^a Average from six replicates.