APPLICATION OF 2,4-DINITROFLUOROBENZENE PRE-COLUMN DERIVATIZATION TO QUANTITATIVE DETERMINATION OF TAURINE AND ITS INTERMEDIATE IN BEVERAGES AND MILK SAMPLES

Abstract

With 2,4-dinitrofluorobenzene–acetonitrile pre-column derivatization, taurine and its intermediate (2-aminoethyl hydrogen sulfate) in beverages and milk samples were determined by high-performance liquid chromatography coupled with diode array detection. Chromatographic separation was performed by isocratic reverse phase model with acetonitrile-phosphate solution (pH 7.0) (19: 81, v/v) as the mobile phase on a C₁₈ column. By precipitation protein and filter, the sample could be directly used for precolumn derivatization and detection. Good linearity was observed in a range of 0.4–500 mg/L for all analytes with the correlation coefficients (r²) ≥ 0.9991. Overall recoveries of the two compounds ranged from 82% to 103% with repeatability (RSD) less than 4.6% at three different spiked levels (5 to 200 mg/L, n = 5). The proposed method offered a good alternative for routine analysis due to its simplicity and at the same time reliability.

Keywords:

• 2-aminoethyl hydrogen sulfate
• beverages
• milk
• precolumn derivatization
• taurine

ACKNOWLEDGMENT

This research was supported by the International Research & Development Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (MEST) of Korea (2010-D00016) and Basic Science Research Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Education, Science and Technology (2012-0005250).

Notes

^a Spiked levels in milk samples.

^b Spiked levels in beverage samples.

—, not determined.