SEPARATION OF (S)-DEHYDROVOMIFOLIOL FROM LEAVES OF NITRARIA SIBIRICA PALL. BY HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY

Abstract

An effective high-speed countercurrent chromatography (HSCCC) method was established for separation and purification of (S)-dehydrovomifoliol from the leaves of Nitraria sibirica Pall. (NS). HSCCC was performed with a two-phase solvent systems composed of n-hexane-ethyl acetate-methanol-water (1:5:1:5, v/v) at a flow rate of 2 mL/min. From 1 g crude sample of NS, 23 mg (S)-dehydrovomifoliol was obtained at purity of 95%. The chemical structure was confirmed by MS, ¹H NMR, and ¹³C NMR. (S)-dehydrovomifoliol was isolated from Nitraria genus for the first time.

Keywords:

• bioactive compound
• (S)-dehydrovomifoliol
• high-speed counter-current chromatography
• Nitraria sibirica Pall.
• separation
• traditional Uighur Medicine

ACKNOWLEDGMENT

This work was financially supported by West Light Foundation of The Chinese Academy of Sciences (No. XBBS201011) and the China National Funds for Distinguished Young Scientists (No. 30925045).