DETERMINATION OF ACETYLSALICYLIC ACID IN PHARMACEUTICAL DRUGS BY TLC WITH DENSITOMETRIC DETECTION IN UV

Abstract

Adsorption thin layer chromatography (NP-TLC) with densitometry has been established for the identification and the quantification of acetylsalicylic acid in tablets. UV densitometry was performed in absorbance mode at 200 nm. The presented method was validated by specificity, range, linearity, accuracy, precision, detection limit, quantitative limit, and robustness. The TLC-densitometric method was also compared with a pharmacopeial UV-spectrophotometric method for the assay of acetylsalicylic acid, and the results confirmed statistically that the NP-TLC-densitometric method can be used as a substitute method. It could be said that the NP-TLC-densitometric method is suitable for the routine analysis of acetylsalicylic acid in quantity control laboratories.

Keywords:

• acetylsalicylic acid
• densitometry
• pharmaceutical analysis
• quantitative analysis
• spectrodensitometry
• spectrophotometry
• TLC

ACKNOWLEDGMENT

This research was financed by the Medical University of Silesia as part of statutory research project KNW-1-004/P/1/0 and KNW-1-001/P/2/0.

Notes

^a AB, Aspirin Protect; PS, Polopiryna S; CA, Cardiopirin; AC, Acard.

^a A, peak area (AU); x, amount (µg spot⁻¹) of drug analyzed; r, correlation coefficient; R, recovery (%); CV, coefficient of variation (%).