Abstract
To overcome some drawbacks of liquid phase microextraction methods, in this study a novel in-tube solid phase extraction coupled with liquid-liquid-liquid extraction (in tube SPE-LLLE) was developed for extraction and determination of naproxen (NAP) as a template drug in plasma sample. In-tube SPE-LLLE is an extraction technique, in which NAP is extracted from the aqueous and plasma samples directly to inner coated surface of a glassy column by polypyrrole that is inoculated with n-octanol. The analyte can be extracted by both adsorption and absorption mechanisms. Using in-tube SPE-LLLE-HPLC-UV, the calibration curve of NAP in plasma was linear in the range from 0.9–450 ng mL−1 with correlation coefficients above 0.9973 and detection limits (S/N = 3) of 0.54 ng mL−1. The relative standard deviations (n = 5) for recovery of 100 ng mL−1of NAP from 1.0 mL of buffer and plasma samples were 3.2 and 5.6%, respectively. This method was successfully applied for analysis of plasma of three patients administrated NAP.
ACKNOWLEDGMENT
We gratefully acknowledge Vice Chancellor for Research and Technology, Kermanshah of Medical Sciences for financial support. This work was performed in partial fulfillment of the requirements for a Pharm. D of Taiebeh Bapirzadeh in Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermansha-Iran.