SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR. TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC

Abstract

An effective high–speed counter–current chromatography (HSCCC) method was established for semi-preparative isolation and purification of bioactive chemical constituents from the Tibetan medicinal plant Pedicularis longiflora var. tubiformis (Klotzsch) P. C. Tsoong. With a two-phase solvent system composed of chloroform–methanol–water (8:4:5, v/v), 40 mg of extract was separated to yield luteolin (12.5 mg), apigenin (9.6 mg), and chrysoeriol (4.8 mg), with purities of 99.3, 98.2, and 98.6%, respectively, as determined by high-performance liquid chromatography (HPLC). The chemical structures of these three components were identified by ¹H NMR and ¹³C NMR.

Keywords:

• apigenin
• chrysoeriol
• flavonoid
• HSCCC
• luteolin
• pedicularis longiflora var. tubiformis (Klotzsch) P. C. Tsoong

ACKNOWLEDGMENT

We are grateful for the National Science and Technology Support Program financial support of China (2007BAC30B04).

Notes

The symbol “∞” means the partition coefficient is too large to be evaluated.