ULTRASONIC-ASSISTED ENZYMATIC IONIC LIQUID-BASED EXTRACTION AND SEPARATION OF FLAVONOIDS FROM CHAMAECYPARIS OBTUSA

Abstract

Flavonoids (quercitrin, myricetin, and amentoflavone) were extracted from Chamaecyparis obtusa by ultrasonic ezymatic ionic liquid-assisted extraction and purified by solid-phase extraction. In this study, flavonoid glycosides in plants were first hydrolyzed using an enzyme. Enzymatic hydrolysis involves the use of cell-wall-degrading enzymes to weaken or rupture the plant cell wall containing the bioactive ingredients, thereby rendering the intracellular bioactive constituents more accessible to extraction. Subsequently, an ionic liquid (IL) was added to the solution to achieve the extraction of flavonoids. Finally, the flavonoids in the extract obtained were purified by solid-phase extraction with ionic liquid-based silica due to multiple interactions between the IL and target compound. Through these steps, quercitrin, myricetin, and amentoflavone were separated without interference with 83.7, 83.6, and 88.3% recovery, respectively. This method provides an effective environmentally friendly approach to extract and separate flavonoids from plants.

Keywords:

• Chamaecyparis Obtusa
• extraction
• flavonoids
• ionic liquids
• separation

ACKNOWLEDGMENT

This research was supported by Basic Science Research Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Education, Science and Technology (2012-0005250).