![Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days]({"type":"image" , "src" : "/sda/110819/TOC-Subject-Sample-2021-Physical-Sciences.jpg"})
Abstract
A novel dispersive liquid–liquid microextraction was developed for the simultaneous determination of furosemide and ethacrynic acid in human urine. Factors affecting the efficiency of microextraction such as the type and volume of extraction solvent, the type and volume of disperser solvent, sample pH, extraction time, and the ionic strength of the sample were investigated. Extraction efficiency was optimal with 50 µL of dichloromethane as the extraction solvent, 1000 µL of acetonitrile as the disperser solvent, and ultrasonic extraction of 5 min. Under the optimum conditions, the enrichment factors of furosemide and ethacrynic acid were above 50 and the limits of detection were 0.047 ng/mL and 0.083 ng/mL. Their linear ranges were 0.5–400 ng/mL for furosemide and 1.2–200 ng/mL for ethacrynic acid. Relative standard deviations (RSDs) were below 4.0%. Average recoveries of spiked compounds were between 84.6 and 92.0%. The proposed method successfully analyzed traces of furosemide and ethacrynic acid in human urine.
ACKNOWLEDGMENT
This study was supported by the Basic Science Research Program through the National Research Foundation (NRF) of Korea funded by the Ministry of Education, Science and Technology (2012-0005250).