PREPARATION OF LUNG CELL MEMBRANE CHROMATOGRAPHY AND THE CHROMATOGRAPHIC AFFINITY STUDY OF 2-METHOXYESTRADIOL AND ITS DERIVATIVES

Abstract

A new model of lung cell membrane chromatography has been established by using a lung cell membrane stationary phase (CMSP), in which lung cell membranes were immobilized on activated silica by the adsorption. Retention characteristics of 2-methoxyestradiol (2-ME) and its synthetic derivatives were investigated under the chromatographic conditions described. The affinities of 2-ME and its synthetic derivatives were expressed by k′ values. The affinity of drugs in the lung cell membrane was consistent with the results of cytotoxicity in vitro. The study indicated that 2-ME had the property of adsorption on the membrane of the lung.

Keywords:

• 2-methoxyestradiol
• affinity
• cell membrane chromatography
• LC
• lung
• stationary phase

ACKNOWLEDGMENT

We thank Chongwei Zhang for providing the materials of 2-ME and its derivatives. We also thank Professor Xiaoni Li for helpful propositions.

This work was supported by a grant from the Experimental Innovation Projects of National Undergraduate Student of China (NO: 101045901). There are no conflicts of interest regarding this paper.