EXTRACTION OF SHIKIMIC ACID FROM CHINESE STAR ANISE USING FLASH COLUMN CHROMATOGRAPHY ON A MOLECULARLY-IMPRINTED POLYMER COLUMN

Abstract

Flash column chromatography prepacked with molecularly imprinted polymers (MIPs) has been successfully used for the extraction of shikimic acid from Chinese star anise. The shikimic acid imprinted polymer with an adsorption capacity of 21.94 µmol g⁻¹ dry polymer and imprinting factor of 4.2 was prepared using the dispersion polymerization method. The capacity of the MIP-Flash column was determined as 23.3 mg g⁻¹ using a frontal chromatography method when 10 m mol L⁻¹ shikimic acid was eluted through the MIP-flash column at 1.7 mL min⁻¹. The crude extract of Chinese star anise loaded on the column was eluted with ethanol/water (40/60, v/v) to remove the impurities, after elution with methanol/water/acetic acid (32/48/20, v/v/v), purified shikimic acid (>95%) was obtained. The MIP-flash column can be reused for at least 4 rounds with capacity lost less than 15%. It was demonstrated that, compared with HPLC, flash column chromatography prepacked with MIPs is a more rapid, feasible, and economical method for the extraction of active ingredients from plants on a large scale based on the technology of molecular imprinting.

Keywords:

• HPLC
• molecularly imprinted technology
• natural product
• polymer chemistry
• proteomics
• separation science and technology

ACKNOWLEDGMENT

Financial support from NSFC (20775007) and National Key Technology R&D Program (2011BAD 30B01) are appreciated. Linguistic advice from Dr. Michale Whitcombe of Cranfield University is acknowledged.