ISOLATION AND PURIFICATION OF FORSYTHOSIDE A AND SUSPENSASIDE A FROM FORSYTHIA SUSPENSA BY HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY

Abstract

Forsythoside A and suspensaside A were isolated and purified from Forsythia suspensa by one step of high-speed counter-current chromatography for the first time using a solvent system of ethyl acetate-n-butanol-methanol-water (4:0.5:0.5:5, v/v). The purities of the two compounds were 98.19% and 98.05%, respectively, as determined by HPLC. Their structures were identified by MS, UV, and NMR analysis. Such a simple and effective method was fairly useful to prepare pure compounds as reference substances for related study on Forsythia suspensa.

Keywords:

• Forsythia suspensa
• forsythoside A
• HSCCC
• isolation
• purification
• suspensaside A

ACKNOWLEDGMENT

This work was supported by the International Scientific and Technological Cooperation Program of China (No: 2009DFA31230) and the Industry-University-Research Cooperation Program from Science and Technology Department of Guangdong Province (No: 2010B090400533).

Notes

Notes. The solvent system 6 was selected. The peak resolution was poor with the solvent systems 1-3, and the elution time was too long with the two solvent systems 4 and 5.