DETERMINATION OF 2-UNDECANONE IN RAT PLASMA AND TISSUE BY A HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD: AN APPLICATION FOR THE PHARMACOKINETIC AND TISSUE DISTRIBUTION RESEARCH

Abstract

The purpose of this study was to establish a reliable high performance liquid chromatography (HPLC) method to investigate the pharmacokinetic and tissue distribution profiles of 2-undecanone. A LC2010AHT HPLC system coupled with UV detector was employed and the separations were performed on a ZORBAX Extend C₁₈ analytical column. The mobile phase was a mixture of 50% acetonitrile and 50% water at a flow rate of 0.7 mL/min with isocratic elution. The method was validated within the concentration range 0.08–5.00 µg/mL for plasma samples, and 0.04–4.00 µg/g for tissue samples, the calibration curves were linear with correlation coefficients >0.99. The limit of detection (LOD) was 0.03 µg/mL or 0.02 µg /g (three times signal/noise ratio). Intra- and inter-day precisions expressed as the relative standard deviation (RSD) for the method were 1.95–3.89% and 2.43–4.23%, respectively. The method recoveries for all samples were >80%. The main pharmacokinetic parameters obtained were t_max = (0.32 ± 0.10) h, C_max = (6.47 ± 1.91) µg/mL, AUC_0→∞ = (9.81 ± 3.11) (µg/mL) · h, and Ka = (5.30 ± 1.73) · h⁻¹. The concentrations of 2-undecanone in liver (5.50 ± 0.26 µg/g) and spleen (3.90 ± 0.22 µg/g) were higher than in the other investigated tissues.

Keywords:

• 2-undecanone
• Houttuynia cordata Thunb. (HC)
• HPLC
• pharmacokinetics
• saururaceae
• tissue distribution

ACKNOWLEDGMENT

This work was supported by a research grant from Xi'an Jiaotong University.

All the authors declare that they have no conflict of interest.

Notes

*the concentration unit is µg/mL.

*the concentration unit is µg/mL.

*the concentration unit is µg/mL.