DETERMINATION OF IMIDACLOPRID RESIDUES IN MULBERRY LEAVES BY QuEChERS AND LIQUID CHROMATOGRAPHY WITH DIODE ARRAY DETECTION

Abstract

A simple and sensitive QuEChERS method for the determination of imidacloprid residues in mulberry leaf was developed using high performance liquid chromatography (HPLC). This method was then evaluated for the residual level and dissipation rate of imidacloprid in mulberry leaves. A maximum initial deposits of imidacloprid on mulberry leaves was found to be 3.047 µg g⁻¹ and 6.490 µg g⁻¹ following two application rates of imidacloprid 17.8 SL at 0.6 and 1.2 mL L⁻¹. The calculated half-life of imidacloprid in mulberry leaves was at recommended dose 3.81 d with a dissipation rate of 76% over 7 d; whereas, in double, the dose was 4.93 days with a dissipation rate of 84% over 10 d.

Keywords:

• dissipation
• HPLC
• imidacloprid
• mulberry leaves
• QuEChERS
• residue

ACKNOWLEDGMENT

The authors are grateful to Department of Agricultural Entomology for providing infrastructural facilities, Tamil Nadu Agricultural University, Tamil Nadu, India, for conduct the experiment.

Notes

SD, standard deviation; RSD, relative standard deviation.

Figures in parenthesis dissipation percentage, BDL, below detection limit; SD, standard deviation.