QUANTITATIVE DETERMINATION OF FOUR ANGIOTENSIN-II-RECEPTOR ANTAGONISTS IN PRESENCE OF HYDROCHLOROTHIAZIDE BY A GRADIENT TECHNIQUE HPLC IN THEIR PHARMACEUTICAL PREPARATIONS

Abstract

Losartan potassium, Valsartan, Telmisartan, and Irbesartan are angiotensin-II-receptor antagonists (ARA II) group, which used in treatment of hypertension alone or in combination with other drugs mainly Hydrochlorothiazide. A gradient HPLC method was developed for the assay of several ARAII in presence of Hydrochlorothiazide. The method was performed by reversed phase high performance liquid chromatography using a mobile phase 0.025 M potassium dihydrogen phosphate (pH 6.0): acetonitrile = 80:20% with detection at 220 nm on an ACE C18 column (250 mm × 4.6 mm, 5 µm) at flow rate 1.5 mL/min in a gradient manner. The proposed method was validated in terms of linearity, accuracy, precision, and limits of detection and quantitation.

Keywords:

• hydrochlorothiazide
• irbesartan
• losartan potassium
• telmisartan
• valsartan

Notes

(Y = a + bC, where C is the concentration of the compound (µg/mL) and Y is the drug peak area).

Where the Tabulated t-values and F -ratios at p = 0.05 are 2.57 and 5.0.