Abstract
A new liquid chromatograpic method has been developed for the chiral separation of the enantiomers of bevantolol hydrochloride. The enantiomers were separated by a Chiralpak IB (250 × 4.6 mm, 5 µm) with a binary solvent mixture of acetonitrile—20 mmol · L−1 KH2PO4 (25/75, v/v; pH 4.5)—at 25°C and detected at 259 nm, with a flow rate of 0.8 mL · min−1. The resolution was 3.90. The effects that organic modifier, type of buffer, mobile phase pH, column temperature, and flow rate had on the enantioselectivity of enantiomers were evaluated. And the structures of the enantiomers of bevantolol hydrochloride were determined.
Notes
Chromatographic condition: the column temperature was at 25°C, and the flow rate was 0.8 mL · min−1; mobile phase was acetonitrile +20 mM KH2PO4 buffer (pH 4.5).
Chromatographic condition: mobile phase was acetonitrile +20 mM buffer, the column temperature was at 25°C, and the flow rate was 0.8 mL · min−1.
Chromatographic condition: mobile phase was acetonitrile +20 mM KH2PO4 buffer, the column temperature was at 25°C, and the flow rate was 0.8 mL · min−1.
Chromatographic condition: mobile phase was acetonitrile—20 mmol · L−1 KH2PO4 (25/75, v/v; pH 4.5)—the flow rate was 0.8 mL · min−1.
Chromatographic condition: mobile phase was acetonitrile—20 mmol · L−1 KH2PO4 (25/75, v/v; pH 4.5)—at 25°C.
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