HPLC DETERMINATION OF VITEXIN-4″-O-GLUCOSIDE IN MOUSE PLASMA AND TISSUES AFTER ORAL AND INTRAVENOUS ADMINISTRATION

Abstract

Vitexin-4″-O-glucoside (VOG) was herein isolated from the leaves of Crataegus pinnatifida Bge. var. major, and then a sensitive and specific high performance liquid chromatography (HPLC) method was developed and validated for studying the pharmacokinetics and tissue distribution of VOG after oral and intravenous administration at a dose of 30 mg/kg. The results indicated that VOG was rapidly and widespreadly distributed throughout the whole body after administration and the oral bioavailability was 11.97 ± 0.66%. The highest VOG level after oral administration was observed in gallbladder, followed by stomach and small intestine. Whilst, the highest VOG level after intravenous administration was found in gallbladder, followed by liver and small intestine.

Keywords:

• HPLC
• oral bioavailability
• oraland intravenous administration
• pharmacokinetics
• tissue distribution
• vitexin-4″-O-glucoside

ACKNOWLEDGMENT

This study was supported by Shenyang Science and Technology Planning Project Foundation (F13-194-9-00), China.

Notes

^a and ^b were the compartmental and non-compartmental approach, respectively.

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