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Journal of Liquid Chromatography & Related Technologies Volume 36, 2013 - Issue 17: SPECIAL ISSUE: THIN LAYER CHROMATOGRAPHY
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Original Articles

COMPARISON OF TWO TLC-DPPH•-IMAGE PROCESSING PROCEDURES FOR STUDYING FREE RADICAL SCAVENGING ACTIVITY OF COMPOUNDS FROM SELECTED VARIETIES OF MEDICAGO SATIVA

Iwona Kowalska Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation – State Research Institute, Pulawy, Poland
,
Łukasz Cieśla Department of Inorganic Chemistry, Medical University of Lublin, Lublin, PolandCorrespondence[email protected]
,
Tomasz Oniszczuk Department of Food Process Engineering, Lublin University of Life Sciences, Lublin, Poland
,
Monika Waksmundzka-Hajnos Department of Inorganic Chemistry, Medical University of Lublin, Lublin, Poland
,
Wiesław Oleszek Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation – State Research Institute, Pulawy, Poland
&
Anna Stochmal Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation – State Research Institute, Pulawy, Poland
Pages 2387-2394 | Published online: 16 Jul 2013
 
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Abstract

TLC-DPPH• test coupled with different image processing procedures have become an interesting technique to screen complex samples for the presence of free radical scavengers. Several procedures have already been published showing the possibility of obtaining quantitative data from TLC plates derivatized with methanolic solution of relatively stable free radical DPPH• (2,2-diphenyl-1-picrylhydrazyl). In this study two different image processing programs, ImageJ and Sorbfil TLC Videodensitometer, have been applied to study direct antioxidant properties of compounds separated from extracts obtained from selected varieties of Medicago sativa. M. sativa var. Radius possessed the greatest amount of compounds with direct antioxidant properties. The activity of the individual compounds has been compared to the activity of rutin, a flavonoid with well recognized antioxidant properties. Neither of the separated compounds was as active as the standard.

Notes

The results are a median value of three measurements.

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