Pharmacokinetic Profiling of a Novel Flavonoid “Viscosine” from Dodonaea viscosa Using High Performance Liquid Chromatography

Abstract

Pharmacokinetic (PK) profiling of a novel flavonoid “Viscosine” was carried out in healthy rabbits following both IV and oral administration. Various PK parameters were investigated using the developed HPLC–UV method. The concentration of the Viscosine in plasma samples following both IV and oral administration was plotted as a function of time on normal and semi-log graph papers. The plasma drug concentration as a function of time profile of the Viscosine following IV bolus administration and oral administration showed the bi-exponential decline that indicates that PK of the Viscosine follows two compartment open model. The Viscosine is rapidly but incompletely absorbed following oral administration from hard gelatin capsules and t_max was achieved just in 0.5 hr (30 min). The mean ± SD of C_max was 0.981 ± 0.021 µg/mL. The very low plasma drug concentration achieved shows that Viscosine has lower permeability following oral administration. Similarly, following IV bolus drug administration, the plasma drug concentration as a function of time curve of Viscosine was a bi-exponential curve that follows two compartment open model. Viscosine is readily distributed and established equilibrium between central and peripheral compartments. Higher volume of distribution (Vd) shows the longer stay in the peripheral compartments. The β-elimination half-life is approximately 3.8 hr. The AUC values also indicate the distribution of the drug in various body fluids.

In summary, the bioavailability of the drug after oral administration (F = 0.32) indicates that Viscosine is a low soluble drug; however, the Vd was higher compared with the IV bolus injection while MRT was about 15.45 hr. These studies indicate that capsules can be used as a suitable dosage form for the administration of Viscosine.

Keywords:

• bioavailability
• HPLC-UV method
• pharmacokinetics
• plasma
• viscosine

Notes

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