SCREENING BACTERIAL AND RADICAL SCAVENGING PROPERTIES OF CHOSEN PLANT EXTRACTS USING THIN-LAYER CHROMATOGRAPHY–DIRECT BIOAUTOGRAPHY

Abstract

Effect directed analysis method is proposed for searching compounds with antibacterial and radical scavenging properties in herbs. The method is based on thin-layer chromatography–direct bioautography of plant extracts and chemical screening using derivatization reagents. Bacillus subtilis and Escherichia coli were used as test bacteria for searching antibacterial properties of plant constituents and DPPH as a reagent for testing radical scavenging properties directly on earlier developed TLC plates. Anisaldehyde-sulfuric acid and natural product/polyethylene glycol 4000 reagent were used for derivatization of terpenoids and polyphenols, respectively.

Keywords:

• B. subtilis
• DPPH test
• E. coli
• effect-directed analysis
• plant screening
• thin-layer chromatography–direct bioautography