Acetylated Ferulenol-Oxy-Ferulenol as a Proposed Marker for Fresh Ferula Toxicity: A Metabolomic Approach

Abstract

Toxicity of fresh Ferula communis (Apiaceae) among cattle in North Jordan is a recently reported problem. Ferula toxicity “Ferulosis” is still ambiguous and not fully understood. Metabolomic approach was applied in the present study to address this problem. Metabolomics based on Attenuated Total Reflectance/Fourier Transformed-Infrared (ATR/FT-IR) results showed that chloroform extract of fresh F. communis has constituents significantly different from those detected in dried plants. Additionally, metabolomics based on HPLC analysis revealed a chromatographic peak with higher concentration in fresh samples. Based on the mass fragments (620, 549, 371, and 274) m/z and neutral losses of acetyl groups (549–505) m/z and (505–461) m/z this compound was identified as acetylated ferulenol-oxy-ferulenol (AFOF).

Keywords:

• chromatography and clustering analysis
• ferulenol
• Ferula communis
• ferulosis
• metabolomics

Notes

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