Investigation of the Composition for a Ternary Solvent System in Tube Radial Distribution Chromatography

Abstract

A tube radial distribution chromatography (TRDC) system was developed based on the tube radial distribution of a ternary solvent system. The composition for the water–hydrophilic organic solvent–hydrophobic organic solvent in TRDC was investigated using phase diagrams, chromatograms, and fluorescence photographs. Mixtures of a hydrophilic organic solvent (acetonitrile, ethanol, methanol, 1-propanol, or 1,4-dioxane) and a hydrophobic solvent (ethyl acetate, hexane, 1-butanol, or chloroform) were examined as the ternary solvent system, which was the carrier solution in TRDC. Water–acetonitrile–chloroform and water–acetonitrile–ethyl acetate systems performed well in TRDC. A model analyte solution of 1-naphthol (NA) and 2,6-naphthalenedisulfonic acid was separated by TRDC using the chloroform solvent system with a range of volume ratios for the solvent components. The elution order of these two compounds could be reversed by changing the solvent system from organic solvent rich to water rich. A mixture of five analytes, NA, Eosin Y, 1-naphthalenesulfonic acid, 2,6-naphthalenedisulfonic acid, and 1,3,6-naphthalenetrisulfonic acid, was also separated using the water–acetonitrile–chloroform solvent system (32:65:3 volume ratio). These compounds eluted in the order given above.

Keywords:

• capillary chromatography
• laminar flow conditions
• separation performance, tube radial distribution chromatography (TRDC)
• tube radial distribution phenomenon (TRDP)
• water–hydrophilic/hydrophobic organic solvent mixture

Notes

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