Optimization of Extraction Techniques and RP-HPLC Analysis of Antidiabetic and Anticancer Drug Mangiferin from Roots of ‘Saptarangi’ (Salacia chinensis L.)

Abstract

Saptarangi (Salacia chinensis L.) is a chief source of mangiferin, which has antidiabetic and anticancer properties. Extraction using continuous shaking extraction (CSE), ultrasonic extraction (UE), microwave assisted extraction (MAE), and steam bath assisted extraction (SBAE) were evaluated for extraction of mangiferin (MF) from roots of S. chinensis. The extracts were analyzed by reverse phase-high performance liquid chromatography (RP-HPLC) and Fourier transform-infra red spectroscopy (FT-IR) analysis. Operational parameters such as different solvents and exposure period were also evaluated. N,N-Dimethyl formamide (DMF 30%, v/v) extracted higher quantity of MF compared to other solvents. The results shows that the extraction of MF by SBAE was more efficient followed by MAE, CSE, and UE methods. Maximum quantity of MF (391.80 ppm) was obtained by SBAE technique and DMF (30%) as a solvent system. SBAE is the easiest and best method for screening of MF from S. chinensis. Furthermore, use of FT-IR validates the RP-HPLC technique for identification and quantification.

Keywords:

• anticancer
• antidiabetic
• FT-IR
• mangiferin
• RP-HPLC
• Salacia chinensis L.

Acknowledgements

Authors are thankful to the Head, Department of Botany and the Principal, Yashavantrao Chavan Institute of Science, Satara for providing necessary laboratory facilities. Authors are also thankful to Dr. V. A. Bapat (INSA & NASc Fellow) Emeritus Scientist, Department of Biotechnology, Shivaji University, Kolhapur, for his critical comments during preparation of this manuscript.