Simultaneous Quantification of Bergenin, Epicatechin, (+)-Catechin, and Gallicin in Bergenia ciliata Using High Performance Liquid Chromatography

Abstract

γBergenia ciliata (Paashanbheda, Saxifragraceae) has been used for centuries in Ayurvedic formulations for a number of ailments related to antiurolithiasis, as an anti-inflamatory, for antitussive activity, and as an antioxidant. In the present communication, four samples of Bergenia ciliata were collected from varying altitudes of Uttrakhand, India, and an analytical HPLC method was developed and validated for the separation and quantification of Bergenin, Epicatechin, (+)-Catechin, and Gallicin (A-D) in Bergenia ciliata. HPLC analysis was carried out onto a reverse phase column (Reodein; RP-C₁₈; size 4.5 × 250 mm, 5.0 µm) with mobile phase started with gradient composition prepared from water:phosphoric acid 99.6:0.3 (component A) and acetonitrile:water:phosphoric acid 79.6:20:0.3 (component B). Scanning wavelength λmax 280 nm was used in the determination of compounds using HPLC. The contents of targeted compounds A–D were found positively correlated with the altitudes of collecting site. However, good correlation was only found in compound A and B contents (r = 0.6892, 0.6398) compared to compound C and D contents (r = 0.1297, 0.3245).

Keywords:

• (+)-catechin
• Bergenia ciliata
• altitudinal variation
• Bergenin
• Epicatechin
• Gallicin
• high performance liquid chromatography