Rapid Identification of Antibacterial Compounds from Turkey Berry by HPLC-Based Metabolomics

Abstract

The aim of this study was to identify antibacterial compounds from Solanum torvum fruit extract by HPLC-based metabolomics method. The antibacterial activity of methanolic extracts, hexane, chloroform, ethyl actetate, and water fractions was assessed by well-diffusion agar method. Only methanol extracts and chloroform fraction showed medium inhibition activity against Staphylococcus aureus. The HPLC chemical profiles of the fractions were then correlated to their antibacterial activity profile by means of Orthogonal Projection to Latent Sructure (OPLS) analysis. Based on Y-related coefficient value, peak of HPLC chromatogram found to be significantly corelated to the antibacterial activity was collected by semi-preparative HPLC and its chemical profile was analyzed by LC-MS. Torvoside A, torvoside G, and torvoside H were found to be dominant in this concentrated fractions; thus, these are the possible compounds responsible for antibacterial activity of the fraction. To confirm this, chemical validation by isolating the compounds and testing its antibacterial activity is required. In this study, the active principals in plant crude extract can be identified rapidly; thus, isolation of the active compounds can be performed in more efficient way.

Keywords:

• antibacteri
• metabolomics
• OPLS
• Solanum torvum
• torvoside
• Turkey berry

Acknowledgments

The contents are the responsibility of Bogor Agricultural University & Texas A&M Borlaug Institute for International Agriculture and do not necessarily reflect the views of USAID or the United States Government. We are grateful to Danang Waluyo, MSc. and Dr. Anis H. Mahsunah from BPPT Biotechnology Center, Serpong Indonesia, for HPLC and LC–MS measurements.