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Abstract
1-Alkyl-3-methylimidazolium-based ionic liquids (tetrafluoroborate, L-(+)-lactate and ethyl sulfate) were used as mobile phase additives to assess the effect of its anionic part on the retention mechanism of perazine in pharmaceutical formulation in reversed-phase high-performance thin-layer chromatography (RP-HPTLC) method. In all cases, significant changes and improvements in the retention factor were observed when ionic liquids were added to the mobile phase. We found that the chromatographic behavior of the basic analyte depends on the utilized ionic liquid as well as its various anions. Enhancement of separation confirms silanol suppressing potency of employed ionic liquids and their positive impact on chromatographic separation of basic drugs. Among selected ionic liquids, the optimum distribution parameters such as shape and quality of spots, high precision, and accuracy in qualitative and quantitative determination characterize the system with [EMIm][BF4] as mobile phase modifier. Our proposed HPTLC method for determination of perazine in oral tablets was also subjected to subsequent validation procedure in accordance with ICH guidelines and proved to be suitable, inexpensive, and convenient method in a pharmaceutical analysis.