Preparative Isolation of Polar Antioxidant Constituents from Abies koreana Using Centrifugal Partition Chromatography Guided by DPPH^•-HPLC Experiment

Abstract

Preparative separation of antioxidant constituents from the leaves of Abies koreana Wilson (Pinaceae) was performed by centrifugal partition chromatography (CPC) with a two-phase solvent system of ethyl acetate-isopropanol-water (9:1:10, v/v) target-guided by DPPH^•-HPLC experiment. In DPPH^•-HPLC experiment, trans-piceid exerted antioxidant activity, which was purified by Diaion-HP 20 macroporous resin chromatography and subsequent CPC. Maltol (98.3 mg), dihydrokaempferol 7-O-β-D-glucopyranoside (230.4 mg), and trans-piceid (165.9 mg) were purified from 2.19 g of crude extract with the purity over 95% by HPLC analysis. The chemical structures of isolated compounds were determined by ¹H, ¹³C NMR, and ESI-MS.

Keywords:

• Abies koreana Wilson
• centrifugal partition chromatography
• DPPH^•-HPLC experiment
• dihydrokaempferol 7-O-β-D-glucopyranoside
• maltol
• trans-piceid