Identification of Flavonoids from Cheilanthes albomarginata Clarke and Their Simultaneous Determination and Quantification by UPLC/DAD Method

Abstract

Cheilanthes albomarginata Clarke, a fern from the Pteridaceae family, is traditionally used to treat peptic ulcer and stomach disorder. Six flavonoid compounds were isolated and identified from the ethyl acetate soluble fraction of this plant. Kumatakenin 5-O-β-glucoside,kaempferol-7-methyl ether, rhamnocitrin 3-O-β-glucoside, and kaempferol 3-O-β-glucoside were isolated for the first time from this plant. UPLC method was developed and used for the simultaneous determination and quantification of the isolated compounds in the methanol extract of the plant. The method was also validated using the parameters: linearity, precision, and accuracy.

Keywords:

• Cheilanthes albomarginata
• flavonoid
• identification
• quantification
• UPLC
• validation

Acknowledgments

We are very thankful to Dr. Hari Prasad Devkota, Kumamoto University, Japan and Prof. Dr. Oh Hyuncheol, Wonkwang University, Republic of Korea for their motivation and valuable information regarding the research work. We also want to acknowledge Mr. Lal Bahadur Thapa, Tribhuvan University, Nepal for the valuable information regarding the plant Cheilanthes albomarginata.