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Original Articles

Multiplexed Detection: Fast Comprehensive Sample Analysis of Tobacco Leaf Extracts Using HPLC with AFT Columns

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Pages 1753-1758 | Published online: 13 Nov 2015
 

Abstract

Detector-based comprehensive screening analysis of complex samples of natural origin using High Performance Liquid Chromatography (HPLC) can be a complicated and time-consuming task. There are a number of ways multidetection characterization can be achieved; however, there are limitations associated with each technique. Active Flow Technology (AFT) in Parallel Segmented Flow (PSF) mode allows for multiplexed detection HPLC analysis within a single injection, whereas maintaining chromatographic performance and allowing the use of multiple destructive detectors to achieve a comprehensive yet efficient screening of a complex sample. In this study, a comprehensive characterization analysis of tobacco leaf extract was carried out through multiplexed detection using a PSF column for the detection of biomolecules by UV-Vis detection, DPPH for reactive-oxygen species (ROS) detection, and mass spectrometry, the latter two detection methods being sample destructive.

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