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ABSTRACT
A new semiautomatic device with two types of horizontal developing chamber for gradient thin-layer chromatography (TLC) that does not require special procedure of chromatographic plate preparation was developed. The new device was applied for separation of test mixture of 10 dyes showing general elution problem. In addition, a general equation for determination of relative position, , of solutes chromatographed under conditions of stepwise gradient elution with one void volume of mobile phase in TLC has been applied. A satisfactory agreement between calculated (by computer program) and experimental values of
has been obtained. In the article, based on obtained results, the advantages and disadvantages of different devices for gradient elution in TLC are discussed.
Acknowledgments
The paper was developed using the equipment purchased within the agreement No. POPW.01.03.00-06-010/09-00 Operational Program Development of Eastern Poland 2007–2013, Priority Axis I, Modern Economy, Operations 1.3. Innovations Promotion.