New and sensitive TLC method to measure trans-resveratrol in Physalis peruviana

ABSTRACT

trans-Resveratrol is a polyphenolic compound belonging to the group of stilbenes. The compound has gained increasing interest over the last few years. In this study, the authors present a new way to measure trans-resveratrol in fresh and dried fruits like goldenberries (Physalis peruviana L.) using a combination of a “Quick, Easy, Cheap, Effective, Rugged and Safe” extraction, a thin-layer chromatography (TLC) separation on TLC silica gel 60 plates (Merck, 1.05554) and chemiluminescence detection. A mixture of ethyl acetate, cyclohexane, and n-butanol (9 + 9+2, v/v) was chosen as mobile phase. The Rf-value for trans-resveratrol is 0.78. The method presented is specific and offers a limit of quantification of 20.3 ng trans-resveratrol per band. Our results indicate that goldenberries seem to be a richer source of trans-resveratrol than red wine.

KEYWORDS:

• Chemiluminescence
• HPTLC
• TLC
• resveratrol
• linear calibration range
• Physalis peruviana

Acknowledgments

The authors express their appreciation to Merck-Company (Darmstadt, Germany), BioStep (Jahnsdorf, Germany), and DESAGA GmbH (Wiesloch, Germany) for their kind support.