ABSTRACT
A rapid and sensitive ultra-performance liquid chromatography–tandem mass spectrometry method with electrospray ionization (UPLC–ESI–MS/MS) for analysis of B-group vitamins in honey has been presented. Aim of this study is the characterization of different types of Turkish honeys according to B-group vitamins. Vitamins were determined in 17 different types of Turkish honey samples by UPLC–ESI–MS/MS. Heather honey samples were distinguished among the studied honeys with the richest vitamin content with 286.10 mg/kg, and it is followed by sunflower honey and thyme honey with the total vitamin contents of 206.01 and 163.27 mg/kg, respectively. The presence of vitamin B1 (thiamine), vitamin B2 (riboflavin), vitamin B3 (nicotinamide, B3N and nicotinic acid, B3H), vitamin B6 (pyridoxine), and vitamin B9 (folic acid) was detected in all the honey samples analyzed. Moreover, vitamin B5 (pantothenic acid) was observed in most of them. Vitamin B12 (cyanocobalamin) and vitamin B7 (biotine) were not detected in the studied honey samples. Turkish honey samples showed efficacious vitamin content for the consumers.