Studies on pharmacokinetics, body distribution, plasma protein binding rate, and excretion of 1-methyl hydantoin in rats in vivo

ABSTRACT

In this paper, plasma concentration, plasma protein binding rate, body distribution, and excretion of both oral and intravenous administration of rats were determined by high performance liquid chromatography (HPLC) combining with UV detector. The blood drug concentration of oral and intravenous administration was summarized. The bioavailability of T_1/2 was approximately 0.75 hr. At the meanwhile, the bioavailability was about 18.84 ± 2.21%. The plasma protein binding rate of 1-methyl hydantoin was about 24.36 ± 0.93%, belonging to low protein binding drug. The result shows that 1-methyl hydantoin can be rapidly distributed in various organs and tissues and quickly eliminated within 6 hr without accumulation in the organs. Its discharge from the urine and feces was 16.58 ± 4.48% and 3.37 ± 0.71%, respectively. All of the results showed that the recovery rate, liner relationship, specificity, stability, and precision of the method were good. The study also proved that 1-methyl hydantoin has been eliminated quite faster in rats.

GRAPHICAL ABSTRACT

KEYWORDS:

• 1-Methyl hydantoin
• HPLC–UV
• Oviductus ranae
• pharmacokinetics

Acknowledgment

Thanks for the support of the National Support Program and Pharmacy of Jilin University to provide various instruments for the experiment.