Dereplication of oligostilbenes in dipterocarpaceous plants using LCMS-ESI-Ion trap-database

ABSTRACT

A method for compound identification based on mass fragmentation patterns was developed to distinguish known oligostilbenes directly in crude plant extracts. Eleven pure oligostilbenes were used as standard compounds, and a pilot database of MS data was established. Seven extracts from Dipterocarpaceous plants; three from Neobalanocarpus heimii, three from Dryobalanops spp. and one from Dipterocarpus semivestitus were used as test materials. Eleven compounds were positively identified in the heartwood extract, nine in the bark, and six in the leaves of Neobalanocarpus heimii, one compound each in Dryobalanops lanceolata, D. rappa and D. aromatica, as well as two in Dipterocarpus semivestitus extract. Identification of all compounds were performed within a short analysis time.

GRAPHICAL ABSTRACT

KEYWORDS:

• Database
• dereplication
• dipterocarpaceae
• HPLC-Ion trap-MS
• oligostilbenes

Acknowledgements

The authors acknowledge the Research Management Centre (RMC), Universiti Teknologi MARA, Malaysia. Appreciation goes to Analytical Unit, Faculty of Pharmacy, UiTM for the instrumentation facilities and the Ministry of Education (MOE) for the research funding under the Fundamental Research Grant Scheme (FRGS). We also want to thank Prof. Emer. Geoffrey A. Cordell, Natural Products Inc., Evanston, IL, USA for reviewing the manuscript.