An efficient approach for enrichment of three isochlorogenic acids from Kuding tea using MCI-GEL resin

ABSTRACT

Kuding tea are used as a traditional tea material and widely consumed in China. Isochlorogenic acids are considered to be one of the major functional ingredients. In this study, nine types of resins including six macroporous resins and three MCI-GEL resins were investigated by adsorption and desorption tests toward the isochlorogenic acids. The HP50SS was selected as the optimal one and the effect of several factors were investigated to make optimization of the adsorption and desorption conditions for enrichment of isochlorogenic acids. As a result, 38.50 mg of refined extract including 31.70 mg total isochlorogenic acids could be separated and enriched from 500.0 mg of crude extract in which the content of total isochlorogenic acids was 8.6% and the recovery of isochlorogenic acids reached 73.4%. Meanwhile, under optimal dynamic adsorption and desorption conditions, the final contents of total isochlorogenic acids increased 9.3-fold when compared to that of crude extract in a scale-up enriched experiment. The results demonstrated that the HP50SS MCI-GEL resin was suitable for purification and enrichment of isochlorogenic acids from Kuding tea. The established protocol was high-efficiency, low-cost and environment-friendly, which could afford a potential approach for industrial applications to enrich and concentrate isochlorogenic acids from Kuding tea.

GRAPHICAL ABSTRACT

KEYWORDS:

• Enrichment
• HPLC
• isochlorogenic acids
• kuding tea
• MCI-GEL resin

Conflict of interest

The authors have declared no conflict of interest.

Additional information

Funding

Funds for this research were provided by grants from National Natural Science Foundation of Hubei Province (No. 2016CFB532) and China (No. 81603331), respectively.