Preparation of analytical columns and HPLC silica-based C₁₈ packing material synthesis from non-toxic silica source

Abstract

Studies about stabilization and lowering the activity of the permanent groups of stationary phases that are used in reversed-phase liquid chromatography (RP-HPLC) considerably increased during the last few decades. In gas and liquid chromatography, silica gels are generally used for as a solid support due to their low price and various particle size products. Moreover, they have a good mechanical stability and swelling resistance. It also provides high efficiency and has different pore sizes that make it easy to make changes in different shapes. In this study, sol-gel is used to synthesis silica gels and during gelation process surfactants are added to attain spherical morphology. Conventional silica gel precursors are predominantly silicone alkoxides with high purity but they are also highly hazardous materials. Therefore, water glass is used as a precursor in our study and C₁₈ groups are linked to silica gels. For C₁₈ linking process, trimethoxyocta decyl silane and trichloroocta decyl silane are tested individually as a cross-linking agent and their performances are compared with various analyses.

Graphical Abstract

Keywords:

• High-performance liquid chromatography
• packing HPLC column
• silica-based C₁₈
• Water glass

Additional information

Funding

Financial supports granted by the both KOSGEB (Small and medium sized enterprises development and support administration) (Project number: 2016/05-4) and Research Foundation of the Istanbul University (Project number: FYO-2017/25934) are gratefully appreciated.