Preparation and characterization of surface-modified Fe₃O₄ magnetic nanoparticles for extraction of flutamide in biological samples using HPLC

Abstract

Flutamide (FLU) loaded onto functionalized magnetite iron oxide nanoparticles were prepared and characterized with scanning electron microscopy, elemental analysis, thermogravimetric analysis, Fourier transform infrared spectroscopy, and energy dispersive spectroscopy techniques. In addition, the effects of parameters of pH, temperature, contact time, and concentration on the adsorption capacity of nanoparticles were evaluated and optimized. The adsorption data were fitted to the Langmuir isotherm and the relevant parameters were inferred. Findings of the study suggested that N-vinylcaprolactam-allylimidazole copolymer coated MNPs are promising carrier for FLU delivery. The solid phase extraction technique was investigated for biological samples. Urine extraction recovery obtained about 91%.

Graphical Abstract

Keywords:

• Biological sample
• drug delivery
• flotamide
• magnetic nanoparticles
• N-vinylcaprolactam