Preparation of thiamphenicol magnetic surface molecularly imprinted polymers for its selective recognition of thiamphenicol in milk samples

Abstract

A magnetic surface molecularly imprinted polymers (MMIPs) with thiamphenicol (TAP) specificity was prepared by using thiamphenicol as a template molecule, methacrylic acid (MAA) as the functional monomer, and silicon dioxide-coated ferriferous oxide (Fe₃O₄ @ SiO₂) as the magnetic carrier. The composition, thermal stability, and magnetic properties of the polymer were characterized by fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), and vibration sample magnetometer (VSM). The results of these studies show that the imprinted polymer layer was successfully applied to the surface of the Fe₃O₄@SiO₂ magnetic material, and that the imprinted particles retain desirable magnetic properties. The MMIPs were applied to extract and quantify thiamphenicol in milk samples. The specific recognition capability of the polymers was affirmed by high performance liquid chromatographic (HPLC) analysis, establishing the limit of detection (LOD) as 10 µg/L and a recovery rate of greater than 85%. A rapid and selective method was established to quantify TAP using MMIPs as a sorbent. The method was used to detect thiamphenicol in milk samples with satisfactory results.

Graphical Abstract

Keywords:

• High performance liquid chromatographic (HPLC)
• magnetic surface molecularly imprinted polymers (MMIPs)
• recognition character
• thiamphenicol

Acknowledgements

Authors would like to thank the National Natural Science Foundation of China for supporting this study.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was financially supported by the National Natural Science Foundation of China [No. 21565001 and No. 31271868], Key Project of North Minzu University [2015KJ30]. Graduate Innovation Project of North Minzu University [No. YCX18077].