Spectrophotometric and liquid chromatographic determination of Zn(II), Ni(II), Fe(II), Co(II), and Cu(II) as metal chelates from vegetable and pharmaceutical samples using 3-hydroxy-5-(hydroxymethyl)-2-methyl-4-pyridine carboxaldehyde-4-phenyl-3-thiosemicarbazone as derivatizing reagent

Abstract

A novel Schiff base chelating ligand 3-hydroxy-5-(hydroxymethyl)-2-methyl-4-Pyridine carboxaldehyde-4-phenyl-3-thiosemicarbazone (Pyridoxal-4-phenyl-3-thiosemicarbazone-PDPTSC) was synthetized and investigated for spectrophotometric and high performance liquid chromatographic (HPLC) separation and determination of Zn(II), Ni(II), Fe(II), Co(II), and Cu(II) as metal chelates on Microsorb C-18 (150 × 4.6 mm i.d, 5 µm) column. A good separation and elution of complexes was achieved isocratically with a mixture of methanol: water: acetonitrile: sodium acetate (1 mM) (65: 30: 4: 1 v/v/v/v) with a flow rate of 1 mL/min and UV detection was at 320 nm. The solvent extraction method was developed for simultaneous determination of metals with detection limits within 9.20–88.0 ng/mL. The developed method was applied for the quantitative determination of metal ions Fe(II), Zn(II) from pharmaceutical preparations, Surbex-Z, Polybion-Z, Fefol, and Fefolvit capsules with percentage recovery within (96.4–98.8%) (RSD% 1.86–4.28) and Co(II) and Cu(II) from vegetable samples carrot, potato, cucumber, and green peas with (RSD% 2.44–3.16) respectively.

Graphical Abstract

Keywords:

• Complexing reagent PDPTSC
• HPLC determination
• metal ions
• pharmaceutical preparation and vegetable samples

Acknowledgements

We like to acknowledge University of Sindh Jamshoro, Pakistan, for providing necessary facilities.

Disclosure statement

The contributing writers of this paper do undertake about the complete nonexistence of any financial ties with any commercial entity in whatsoever manner that may bring about a conflict of interests.