Analysis of three emulsifiers of diglyceride in milk by ultra performance liquid chromatography – Evaporative light scattering detector

Abstract

In this paper, a new method was developed for the determination of glyceryl dilinoleate, 1,3-dipalmitate glyceride, and glyceryl 1,3-distearate in milk by ultra-performance liquid chromatography—evaporative light scattering detector (UPLC-ELSD). The analytes were extracted with acetone, dried by nitrogen and then dissolved in isopropanol. Under the optimized conditions, glyceryl dilinoleate (10–100 mg/L), glyceryl 1,3-dipalmitate (10–100 mg/L) and glyceryl 1,3-distearate (10–100 mg/L) showed a good linear relationship (R²>0.997), with LODs and LOQs in the range of 3.15–3.19 mg/L and 10.30–10.50 mg/L, respectively. The recoveries of the analytes in the milk were in the range of 72.5% − 127.5%, with the relative standard deviations (RSD) (n = 6) less than 6.0%. All the results indicated that the method was suitable for the determination of glyceryl dilinoleate, glyceryl 1,3-dipalmitate and glyceryl 1,3-distearate in milk.

Graphical Abstract

Keywords:

• 1,3-Dipalmitate glyceride
• glyceryl 1,3-distearate
• glyceryl dilinoleate
• liquid chromatography-evaporative light scattering
• milk
• ultra performance

Disclosure statement

The authors declare that they have no conflict of interests in relation to the work described.

Additional information

Funding

The authors are grateful for the financial sponsoring of National Key Research and Development Project [No.2018YFC1604306], Science and Technology Innovation Platform Project of Jiangxi Province [No.20192BCD40001].