Metabonomics comparison of coronary heart disease with and without turbid phlegm syndrome

Abstract

Objectives

To help understand the pathogenesis of coronary heart disease (CHD) by doing blood metabonomics comparison on CHD patients with phlegm-turbidity syndrome and without syndrome, and healthy controls.

Methods

Ninety patients were involved. Among them, 60 patients with CHD were divided into the coronary heart disease with turbid phlegm syndrome group (HZTZ) and the coronary heart disease without turbid phlegm syndrome group (NHZTZ). The additional 30 subjects were the healthy controls (JKZ). The serum endogenous metabolites were determined and analyzed by Liquid chromatography—Mass spectrometry (LC-MS).

Results

In positive ion mode, there were three identical differential metabolites among the three groups: N2-ethyl-n4-isopropyl-6-(methylthio)-1,3,5-triazine-2,4-diamine, LysoPC (18:2), and methyl palmitate. In negative ion mode, two identical metabolites were identified, which were LysoPC (17:0) and fatty acyl ester of hydroxy fatty acids (FAHFA) (17:1/14:1). These five metabolites of the HZTZ group were higher than those in NHZTZ group and healthy controls. The results identified perturbations in the areas, such as phenylalanine, tyrosine, and tryptophan biosynthesis, starch and sucrose metabolism, carbohydrate digestion and absorption, and so on.

Conclusions

Metabonomics analysis of serum in patients with CHD with turbid phlegm syndrome uncovered the metabolic disorders of saturated and unsaturated fatty acids in comparison to the patients without turbid phlegm syndrome.

Graphical Abstract

Keywords:

• Coronary heart disease (CHD) with turbid phlegm syndrome
• coronary heart disease (CHD) without turbid phlegm syndrome
• metabonomics
• metabolism of saturated fatty acids
• metabolism of unsaturated fatty acids

Acknowledgments

We thank LetPub (www.letpub.com) for linguistic assistance and pre-submission expert review.

Author’s contributions

Guarantor of integrity of the entire study and study concepts: Xiaofeng Wang, Jing Wang, and Pengcheng Zhu; study design: Xiaofeng Wang and Jing Wang; definition of intellectual content: Pengcheng Zhu; literature research: Guligena Sawuer and Xueqin Zhai; clinical studies: Min Jiang; experimental studies: Hui Fan; data acquisition and data analysis: Yaonan Du; statistical analysis: Min Jiang; manuscript preparation: Jing Wang; manuscript editing and manuscript review: Xiaofeng Wang and Jing Wang.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by National Natural Science Foundation of China (Grant number 81760794).