Abstract
A highly sensitive, accurate, and robust LC-MS/MS method was developed and validated for the simultaneous determination of ornidazole (ODZ) and miconazole (MCZ) in rat plasma using metronidazole (MTR) as an internal standard (IS). The analyte and IS were extracted from plasma by precipitating protein with acetonitrile and were chromatographed. The mobile phase was acetonitrile and 0.1% formic acid (75:25 v/v). The total run time was 3 min, and the injection volume was 5.0 μL. Multiple reaction monitoring (MRM) mode was used, in which the transitions of m/z 417.10→m/z 160.90 for MCZ, m/z 220.10→m/z 127.90 for ODZ, and m/z 172.10→m/z 128.1 for IS were monitored. The linearity range of MCZ was 0.15 − 250.0 ng/mL and 3.0-250.0 ng/mL for ODZ. The lower limits of quantification (LLOQ) were found to be 0.05 ng/mL for MCZ and 1.0 ng/mL for ODZ in rat plasma. Recoveries of MCZ ranged between 75.33% − 94.25% and ODZ ranged between 75.33% − 95.54%. The quality control sample accuracy and precision (intra-day and inter-day) were −1.25% to 1.68% and 3.01% to 6.44% for MCZ and −1.31% to 1.88% and 3.34% to 6.98% for ODZ, respectively. The analytes and IS were stable during all sample storage and analysis procedures.
Acknowledgments
The authors would like to extend their sincere appreciation to the Researchers Supporting Project Number (RSP2023R301), King Saud University, Riyadh, Saudi Arabia. The authors would like to thank the Birla Institute of Technology, Mesra, Ranchi, India; and the Management of Bio-analytical Technologies (India) Pvt. Ltd. for providing the necessary facilities to carry out this research.
Disclosure statement
No potential conflict of interest was reported by the author(s).