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Abstract
The application of a novel high performance liquid chromatographic (HPLC) technique for the determination of histamine in urine, cerebral spinal fluid (CSF), and brain tissue from rat is been reported. The determination of histamine in these biological samples was accomplished by injecting the samples into a Dionex Bio-HPLC system. Using this method 780 pmols/5 μll, 25.8 pmols/5 μl, 819 pmoles/mg of tissue/5 μl of histamine in urine, CSF, and brain tissue, respectively, were detected. The retention time for histamine is less than 5 minutes. This reported method proves to be suitable for the quantitation of histamine in urine, cerebral spinal fluid, and brain tissue.