Determination of Oxolinic Acid in Seawater, Marine Sediment, and Japanese Oyster (Crassostrea Gigas) by High-Performance Liquid Chromatography

Abstract

A reversed-phase high-performance liquid chromatographic method was developed to detect oxolinic acid in seawater, marine sediment, and japanese oyster (Crassostrea gigas). Seawater was analysed after filtration and centrifugation. Sediment and oyster tissues were respectively analysed after liquid-phase and solid-phase extraction. Linearity and precision were checked over the concentration range 0.05–2.50 μg/mL or μg/g. Limits of detection and determination were respectively 0.01 and 0.04 μg/mL or μg/g. Recoveries of oxolinic acid were 102.1% from seawater, 68.1% from sediment and 88.3% from oyster. Oxolinic acid concentrations in seawater, sediment and oyster spiked at 0.50 μg/mL or μ.g/g were stable at −20°C for 60 days.