Comparison of Methods Evaluating Lipoperoxidation in Plasma of Malaria Patients

Abstract

Lipid peroxidation is believed to be involved in malaria and plasma of malaria patients may be damaged by reactive oxygen soecies This study compares determinations of malondialdehyde ifiDA), vitamins A and E by high performance liquid chromatography, thiobarbituric acid reactive substances (TBA-RS) by fluorometric analysis and fatty acids by gas chromatography. During malarial infection, polyunsaturated fatty acids (PUFA), vitamins A and E decreased significantly when TBA-RS increased significantly. However, there was no significant change >n total or MDA bound to amino-groups. On the other hand, there was no correlation between TBA-RS production and PUFA decrease or between TBA-RS production and vitamin A or E consumption. These results support the concept that other compounds such as proteins and cholesterol may be involved in the formation of these TBA-RS.