Comparison of Amino Acid Separations on High Performance Silica Gel, Cellulose, and C-18 Reversed Phase Layers and Application of HPTLC to the Determination of Amino Acids in Biomphalaria Glabrata Snails

Abstract

The separation of 18 amino acids on high performance silica gel, cellulose, and reversed phase bonded silica gel plates has been compared. Zones were detected with ninhydrin reagent. The ability to identify amino acids in Biomphalaria glabrata snail hemolymph and digestive gland-gonad complex (DGG) by HPTLC was studied, and alanine and aspartic acid were quantified in hemolymph and DGG by scanning densitometry. Analytical results were compared to those reported earlier using column ion exchange and reversed phase liquid chromatography and paper chromatography.