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Abstract
In the paper two ways of using thin-layer chromatographic data for mobile phase optimization in column chromatography have been presented. The first method is connected with experimental correlations between thin-layer and column retention parameters. This moderately simple method needs to determine the correction parameter to transfer experimental thin-layer data to liquid column technique. In the paper the effects of mobile phase kind and cocentration, as well as chromatographed solute character on this parameter have been presented. The other method is based on thermodynamic description of adsorption systems with mixed mobile phases, i.e., adsorbent + binary solvent + solute systems. This treatment is very precise, but it needs a lot of experimental thin-layer data.
In the paper the possibility of using TLC experimental data for the choice of optimal mobile phase kind and composition in two-component solvents and some organic model solutes has been presented.