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Abstract
The retention behavior of five 17-oxosteroids (dehydroepiandrosterone, epiandrosterone, androsterone, 5β -androsterone, and 5β -androstane-3β ol-17-one) derivatized with 5-dimethylamino-1-naphthalenesulfonic hydrazide, 4-(N,N-dimcthylaminosulfonyl)-7-hydrazino-2,1,3- benzoxadiazole or p-nitrophenylhydrazine are examined using reversed-phase high-performance liquid chromatography. Inclusion chromatography using cyclodextrin as a mobile phase additive is also used for this purpose and found effective in separating the isomeric derivatized 17-oxosteroids.