![Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days]({"type":"image" , "src" : "/sda/110819/TOC-Subject-Sample-2021-Physical-Sciences.jpg"})
Abstract
A stereospecific high-performance liquid chromatographic method for the simultaneous determination of the four stereoisomers of 4-hydroxytertatolol, an active metabolite of tertatolol, in human and rat urine is described. The method is based on solid-phase extraction of urine followed by derivatization with S(+)-naphtylethylisocyanate. The four stereoisomers were resolved by reversed phase high-performance liquid chromatography and detected by fluorescence (λexcitation = 210 nm, no cutoff emission filter). The analytical method is suitable for the simultaneous determination of the four stereoisomers of 4-hydroxytertatolol in rat and human urine down to 22.0 ng/ml. Preliminary results in rat suggest stereoselective hydroxylation of tertatolol enantiomers.